Fungicide assay by spore germination in shaker flasks.

Since the introduction in 1933 (Kluyver and Perquin, 1933) of the shaker flask method of culturing fungi this technique has found widespread use in fungus physiology (Foster, 1949). It was used for spore germination studies by Davies et al. (1948) and Mandels and Norton (1948). The measurement of antifungal activity, however, has been accomplished by the more conventional methods making use of agar plates, hanging drops, moist chambers, and more elaborate techniques (Horsfall, 1946). This paper describes the assay of fungicides by spore germination in shaker flasks.